Lugea A, Mourelle M, Guarner F, Domingo A, Salas A, Malagelada J R
Digestive System Research Unit, Hospital General Vall d'Hebron, Barcelona, Spain.
Gastroenterology. 1994 Sep;107(3):720-7. doi: 10.1016/0016-5085(94)90119-8.
BACKGROUND/AIMS: Surfactant phospholipids impede diffusion of acid through the gastric mucus, but their relevance in the defense of the duodenum against luminal acid is not known.
Duodenal resistance to acid was tested in anesthetized rats by instillation of HCl using a tube implanted in the proximal duodenum. The effects of a detergent (Brij 35; Sigma, St. Louis, MO) and a lipid mixture flushed through the luminal surface on duodenal resistance to acid were studied. The lipid content in the mucus and the effects of acid, prostaglandins, and indomethacin on the lipid layer were also analyzed.
Instillation of 100 mumol HCl or 5 micrograms/kg 16,16-dimethyl prostaglandin E2 increased resistance to acid, preventing duodenal lesions induced by 500 mumol HCl. However, 100 mumol HCl or 16,16-dimethyl prostaglandin E2 did not prevent lesions induced by 500 mumol HCl in rats undergoing perfusions with 5% Brij 35. Indomethacin suppressed acid-induced protection. A mixture of tripalmitin and dipalmitoyl-phosphatidylcholine protected against 500 mumol HCl, and the effect was also observed in rats receiving indomethacin. Finally, 100 mumol HCl increased the phosphatidylcholine content in the duodenal mucus but not in rats receiving 5% Brij 35 or indomethacin.
Surface-active phospholipids are critical for adaptive cytoprotection to acid in the rat duodenum.
背景/目的：表面活性磷脂可阻碍酸通过胃黏液扩散，但它们在十二指肠抵御腔内酸方面的相关性尚不清楚。
通过将一根植入十二指肠近端的导管注入盐酸，在麻醉大鼠中测试十二指肠对酸的抵抗力。研究了一种去污剂（Brij 35；西格玛，密苏里州圣路易斯）和通过腔表面冲洗的脂质混合物对十二指肠抗酸能力的影响。还分析了黏液中的脂质含量以及酸、前列腺素和吲哚美辛对脂质层的影响。
注入100微摩尔盐酸或5微克/千克16,16-二甲基前列腺素E2可增加对酸的抵抗力，预防由500微摩尔盐酸诱导的十二指肠损伤。然而，在接受5% Brij 35灌注的大鼠中，100微摩尔盐酸或16,16-二甲基前列腺素E2并不能预防由500微摩尔盐酸诱导的损伤。吲哚美辛抑制酸诱导的保护作用。三棕榈精和二棕榈酰磷脂酰胆碱的混合物可预防500微摩尔盐酸，在接受吲哚美辛的大鼠中也观察到了这种效果。最后，100微摩尔盐酸增加了十二指肠黏液中的磷脂酰胆碱含量，但在接受5% Brij 35或吲哚美辛的大鼠中未增加。
表面活性磷脂对大鼠十二指肠对酸的适应性细胞保护至关重要。
