Isenberg J I, Smedfors B, Johansson C
Gastroenterology. 1985 Jan;88(1 Pt 2):303-7. doi: 10.1016/s0016-5085(85)80184-0.
The purpose of this study was to examine the effect of graded doses of intraluminal HCl and prostaglandin E2 on proximal duodenal bicarbonate secretion in unanesthetized rats. Basal bicarbonate secretion was approximately 14 mumol/cm . h and increased significantly in a dose-related manner to 50, 100, and 150 mM HCl as follows: 22.0 +/- 1.5, 29.2 +/- 4.0, and 42.0 +/- 4.3 mumol/cm . h, respectively (mean +/- SE). After a single 5-min infusion of 150 mM HCl, duodenal bicarbonate secretion remained significantly above basal for almost 3 h. The responses to 100 mM HCl and 10(-4) M prostaglandin E2 were similar (29.2 +/- 4.0 and 26.9 +/- 4.4 mumol/cm . h, respectively). Indomethacin (4 mg/kg, intraperitoneally) suppressed the bicarbonate response to 150 mM HCl; whereas the response to 10(-4) M prostaglandin E2 was unaltered. These studies in the in vivo rat indicate that (a) graded doses of intraluminal HCl produced graded increases in proximal duodenal bicarbonate secretion; (b) the bicarbonate output in response to a 5-min infusion of HCl persisted for almost 3 h; and (c) the bicarbonate response to HCl was essentially abolished by indomethacin pretreatment, suggesting that endogenous prostaglandins are at least in part involved in acid-induced proximal duodenal bicarbonate secretion.
本研究的目的是检测不同剂量的腔内盐酸（HCl）和前列腺素E2对未麻醉大鼠近端十二指肠碳酸氢盐分泌的影响。基础碳酸氢盐分泌量约为14 μmol/cm·h，随着HCl剂量从50、100增至150 mM，碳酸氢盐分泌量显著增加，具体如下：分别为22.0±1.5、29.2±4.0和42.0±4.3 μmol/cm·h（均值±标准误）。在单次输注150 mM HCl 5分钟后，十二指肠碳酸氢盐分泌在近3小时内仍显著高于基础水平。对100 mM HCl和10⁻⁴ M前列腺素E2的反应相似（分别为29.2±4.0和26.9±4.4 μmol/cm·h）。吲哚美辛（4 mg/kg，腹腔注射）抑制了对150 mM HCl的碳酸氢盐反应；而对10⁻⁴ M前列腺素E2的反应未改变。这些在活体大鼠中的研究表明：（a）腔内不同剂量的HCl使近端十二指肠碳酸氢盐分泌呈剂量依赖性增加；（b）对HCl输注5分钟的碳酸氢盐输出持续近3小时；（c）吲哚美辛预处理基本消除了对HCl的碳酸氢盐反应，提示内源性前列腺素至少部分参与了酸诱导的近端十二指肠碳酸氢盐分泌。
