双芽巴贝斯虫可溶性沉淀抗原的提取及其诊断初步应用
Extraction and preliminary use for diagnosis of soluble precipitating antigen from Babesia bigemina.
作者信息
Goldman M, Bukovsky E
出版信息
J Protozool. 1975 May;22(2):262-4. doi: 10.1111/j.1550-7408.1975.tb05863.x.
Abstract
Soluble antigen from bovine blood with a Babesia bigemina parasitemia of 5-6% formed 4 precipitin lines in gel diffusion tests with antiserum from infected cattle. Antigen was obtained from plasma and hemolysate by elution from DEAE cellulose columns and (NH4)2SO4 precipitation as well as from washed parasite-erythrocyte stroma by sonication or freeze-thawing. About 5 ml of antigen suitable for diagnostic tests could be extracted from 200 ml of infected blood.
摘要
来自感染牛血液的可溶性抗原，其巴贝斯虫双芽种血症为5 - 6%，在与感染牛抗血清的凝胶扩散试验中形成了4条沉淀线。抗原通过从DEAE纤维素柱上洗脱、硫酸铵沉淀从血浆和溶血产物中获得，以及通过超声处理或冻融从洗涤过的寄生虫 - 红细胞基质中获得。从200毫升感染血液中可提取出约5毫升适用于诊断测试的抗原。
Zotero 插件