Mourey M S, Quadro L, Panariello L, Colantuoni V
Dipartimento di Biochimica e Biotecnologie Mediche, Facolta' di Medicina e Chirurgia, Universita' degli Studi di Napoli Federico II, Italy.
J Cell Physiol. 1994 Sep;160(3):596-602. doi: 10.1002/jcp.1041600323.
The expression of the gene coding for retinol-binding protein has been studied in a system of cultured human hepatoma cells exposed to retinoids. We report that the gene is positively modulated by retinol and retinoic acid in a time- and dose-dependent fashion. The stimulation at the mRNA level is paralleled by an increase of the corresponding protein that is secreted in the presence of the physiological ligand. An RBP-CAT chimeric gene, introduced by transfection, is also responsive to the treatment, showing the gene dose-dependency as the endogenous gene. These results demonstrate that retinoids up-regulate the RBP gene and that the control takes place at transcriptional level.
在一个培养的人肝癌细胞系统中，研究了编码视黄醇结合蛋白的基因表达，该系统暴露于类视黄醇。我们报告该基因受到视黄醇和视黄酸以时间和剂量依赖性方式的正向调节。mRNA水平的刺激伴随着相应蛋白质的增加，该蛋白质在生理配体存在的情况下分泌。通过转染引入的RBP-CAT嵌合基因也对该处理有反应，显示出与内源基因一样的基因剂量依赖性。这些结果表明类视黄醇上调RBP基因，并且这种调控发生在转录水平。
