abou Hassan M M, Seleem M A, Etewa S E
Department of Parasitology, Faculty of Medicine, Zagazig University, Egypt.
J Egypt Soc Parasitol. 1994 Aug;24(2):363-70.
This work was designed to study the relation between cellular and humoral immune responses in experimentally infected albino rats with T. spiralis larvae over a period of four weeks. Cell-mediated immune response (measured by lymphoblast transformation test) revealed a marked lymphoblast response during the intestinal phase, but the reaction became irregular with larval encystation in muscles. Humoral immune response was estimated by both of micro-and Dot-ELISA techniques. It was obvious that significant higher O.D. readings and dot color intensities were observed in the muscular phase than in intestinal phase. The results proved that micro-ELISA had the least accuracy (66.6%) and poorest specificity (81.25%), while Dot-ELISA showed the best sensitivity (100%) and highest specificity (87.5%). Lymphoblast transformation was as specific as Dot-ELISA.
本研究旨在探讨实验感染旋毛虫幼虫的白化大鼠在四周内细胞免疫和体液免疫反应之间的关系。细胞介导的免疫反应（通过淋巴细胞转化试验测量）显示，在肠道期有明显的淋巴细胞反应，但随着幼虫在肌肉中包囊化，反应变得不规则。通过微量ELISA和斑点ELISA技术评估体液免疫反应。很明显，在肌肉期观察到的光密度读数和斑点颜色强度显著高于肠道期。结果证明，微量ELISA的准确性最低（66.6%），特异性最差（81.25%），而斑点ELISA显示出最佳的敏感性（100%）和最高的特异性（87.5%）。淋巴细胞转化与斑点ELISA一样具有特异性。
