Strelow L I, Laycock K A, Jun P Y, Rader K A, Brady R H, Miller J K, Pepose J S, Leib D A
Department of Ophthalmology and Visual Sciences, Washington University School of Medicine, St Louis, Missouri 63110.
J Gen Virol. 1994 Sep;75 ( Pt 9):2475-80. doi: 10.1099/0022-1317-75-9-2475.
The promoters of the latency-associated transcripts (LATs) of herpes simplex virus type 1 (HSV-1) strains KOS and McKrae were compared to examine their influence upon the reactivation phenotypes of these two strains. Unlike strain KOS, McKrae is readily reactivable using in vivo reactivation models. We found greater than 96% sequence conservation between KOS and McKrae in the LATs promoter region, and both promoters showed equivalent basal and inducible activities. An inter-strain recombinant (termed MK13) was constructed in which the LATs promoter of HSV-1 McKrae was recombined into the background of HSV-1 strain KOS. In a murine u.v. light-induced reactivation model, virus shedding was detected by eye swabbing in two of 44 (5%) mice infected with KOS, 20 of 42 (48%) mice infected with McKrae and none of 45 (0%) mice infected with MK13. These data show that the LATs promoters of these viruses are structurally and functionally similar and that transfer of the LATs promoter from McKrae into KOS is insufficient to confer a reactivatable phenotype.
比较了单纯疱疹病毒1型（HSV-1）毒株KOS和McKrae的潜伏期相关转录本（LATs）启动子，以研究它们对这两种毒株再激活表型的影响。与毒株KOS不同，McKrae使用体内再激活模型很容易被再激活。我们发现KOS和McKrae在LATs启动子区域的序列保守性大于96%，并且两个启动子都表现出相当的基础活性和诱导活性。构建了一种株间重组体（称为MK13），其中HSV-1 McKrae的LATs启动子被重组到HSV-1毒株KOS的背景中。在小鼠紫外线诱导的再激活模型中，通过眼拭子检测病毒脱落情况，结果显示，在感染KOS的44只小鼠中有2只（5%）、感染McKrae的42只小鼠中有20只（48%）检测到病毒脱落，而感染MK13的45只小鼠中无一（0%）检测到病毒脱落。这些数据表明，这些病毒的LATs启动子在结构和功能上相似，并且将McKrae的LATs启动子转移到KOS中不足以赋予可再激活表型。
