Yu X, Barnhill R L, Graves D T
Department of Oral Biology, Boston University School of Graduate Dentistry, Massachusetts.
Lab Invest. 1994 Aug;71(2):226-35.
Monocyte chemoattractant Protein-1 (MCP-1) is a potent chemotactic factor for monocytes. Because many inflammatory dermatoses are characterized by mononuclear cell infiltrates, it is reasonable to postulate that MCP-1 might be involved in their pathogenesis. To date, no in vivo studies have been published concerning the expression of MCP-1 in this context. The aim of this study was to elucidate the expression of MCP-1 in human inflammatory skin diseases which are thought to involve delayed type hypersensitivity reactions.
Expression of MCP-1 was examined in normal skin and three classes of inflammatory skin reactions by immunohistochemistry experiments utilizing a monospecific MCP-1 antiserum. The distribution of monocytes/macrophages and T lymphocytes was determined by immunohistochemistry using antibodies to specific cell surface markers.
Immunostaining with MCP-1 antiserum demonstrated strong MCP-1 expression in lichenoid dermatitis, dermal hypersensitivity reactions, and spongiotic dermatitis. In contrast, normal skin showed minimal MCP-1 expression in the dermis. The cell types displaying MCP-1 expression were endothelial cells of dermal microvessels that were surrounded by lymphocytic infiltrates and monocytes/macrophages at the periphery of the perivascular infiltrates. Occasionally, MCP-1-positive mononuclear cells were present both in the infiltrates and in a diffuse pattern in the surrounding dermis. Keratinocytes were found to produce MCP-1 constitutively in normal skin and in inflamed conditions. The pattern of MCP-1 expression was similar to the pattern observed for monocyte/macrophage distribution, whereas the pattern of MCP-1 expression was different from the pattern of T lymphocyte distribution.
We observed an enhanced expression of MCP-1 in inflammatory skin conditions. The expression of MCP-1 provides a mechanistic explanation for the increased recruitment of monocytes/macrophages in cell-mediated immune response such as delayed type hypersensitivity reactions in the skin.
单核细胞趋化蛋白-1（MCP-1）是一种对单核细胞有强大趋化作用的因子。由于许多炎症性皮肤病的特征是单核细胞浸润，因此推测MCP-1可能参与其发病机制是合理的。迄今为止，尚未有关于MCP-1在这种情况下表达的体内研究发表。本研究的目的是阐明MCP-1在被认为涉及迟发型超敏反应的人类炎症性皮肤病中的表达。
通过使用单特异性MCP-1抗血清的免疫组织化学实验，检测正常皮肤和三类炎症性皮肤反应中MCP-1的表达。使用针对特定细胞表面标志物的抗体，通过免疫组织化学确定单核细胞/巨噬细胞和T淋巴细胞的分布。
用MCP-1抗血清进行免疫染色显示，苔藓样皮炎、皮肤超敏反应和海绵状皮炎中MCP-1表达强烈。相比之下，正常皮肤在真皮中显示出最小的MCP-1表达。显示MCP-1表达的细胞类型是真皮微血管内皮细胞，其周围有淋巴细胞浸润，血管周围浸润的外周有单核细胞/巨噬细胞。偶尔，MCP-1阳性单核细胞既存在于浸润中，也以弥漫性模式存在于周围真皮中。发现角质形成细胞在正常皮肤和炎症状态下都组成性地产生MCP-1。MCP-1的表达模式与单核细胞/巨噬细胞分布的模式相似，而MCP-1的表达模式与T淋巴细胞分布的模式不同。
我们观察到炎症性皮肤疾病中MCP-1表达增强。MCP-1的表达为细胞介导的免疫反应（如皮肤迟发型超敏反应）中单核细胞/巨噬细胞募集增加提供了一种机制解释。
