Ansari K A, Wells K, Vatassery G T
Neurology. 1975 Jul;25(7):688-92. doi: 10.1212/wnl.25.7.688.
CSF samples were obtained from 44 multiple sclerosis patients during exacerbation and from 50 age and sex-matched non-multiple sclerosis controls. In an attempt ot select the most suitable test for diagnosis of multiple sclerosis, each sample was analyzed by three methods: (1) electrophoresis, (2) electroimmunodiffusion, and (3) radial immunodiffusion (RID). Electrophoresis, electroimmunodiffusion, and radial immunodiffusion yielded positive results in 50, 55, and 59 percent of the multiple sclerosis patients respectively. Electrophoresis, electroimmunodiffusion, and radial immunodiffusion yielded 10, 12, and 8 percent false positives (beyond mean +/- 1 S.D.) respectively. Thus the three tests did not differ much with regard to yield of true or false positive results. When factors such as simplicity, time and equipment involved, and the interval between the test and results are taken into account, radial immunodiffusion appears to be most suitable for an avverage CSF diagnostic laboratory. For a laboratory with special interest in multiple sclerosis, agarose gel-electrophoresis of concentrated CSF appears to be the test of choice.
在病情加重期间，从44例多发性硬化症患者中采集脑脊液样本，并从50例年龄和性别匹配的非多发性硬化症对照者中采集样本。为了尝试选择最适合诊断多发性硬化症的检测方法，每个样本都通过三种方法进行分析：（1）电泳，（2）电免疫扩散，以及（3）放射免疫扩散（RID）。电泳、电免疫扩散和放射免疫扩散分别在50%、55%和59%的多发性硬化症患者中产生阳性结果。电泳、电免疫扩散和放射免疫扩散分别产生10%、12%和8%的假阳性结果（超过均值±1个标准差）。因此，这三种检测在真阳性或假阳性结果的产生方面差异不大。当考虑到诸如操作简易性、所需时间和设备以及检测与结果之间的间隔等因素时，放射免疫扩散似乎最适合一般的脑脊液诊断实验室。对于对多发性硬化症有特殊兴趣的实验室，浓缩脑脊液的琼脂糖凝胶电泳似乎是首选检测方法。
