Fisseler-Eckhoff A, Becker T, Sudhoff H, Müller K M
Institute of Pathology, Berufsgenossenschaftliche Kliniken, Bergmannsheil, Bochum-University Clinic, FRG.
Pathol Res Pract. 1994 Apr;190(4):389-93. doi: 10.1016/S0344-0338(11)80412-7.
Silver staining of argyrophil nucleolar organizer regions (AgNORs) has become a valuable method to assess cell kinetics in paraffin sections and separate benign from malignant lesions. As a putative technique for the distinction between regenerative and preneoplastic alterations of the bronchial mucosa we employed the Ag-staining method, evaluating the number of dots/nucleus and shape of the dots. Dots/nucleus were counted by by standardized methods, and mean and standard deviation were calculated. Comparative investigations were performed in bioptically obtained specimens of normal bronchial epithelium (1.94 +/- 0.1 dots/nucleus, n = 12), basal cell hyperplasia (2.22 +/- 0.13 dots/nucleus, n = 15), goblet cell hyperplasia (2.38 +/- 0.16 dots/nucleus, n = 17), squamous cell metaplasia (2.58 +/- 0.17 dots/nucleus, n = 25), squamous cell metaplasia with mild and moderate dysplasia (2.50 +/- 0.5 dots/nucleus, n = 15), squamous cell metaplasia with severe dysplasia and carcinoma in situ (3.10 +/- 0.47 dots/nucleus, n = 17), micropapillomatosis (2.20 +/- 0.21 dots/nucleus, n = 13) and early squamous cell carcinoma of the bronchus (4.40 +/- 0.74, n = 13). In preneoplastic lesions a variability in form and size of the dots was found. Though showing an increase in the number of dots/nucleus coincidentally with the degree of proliferative activity, the AgNOR method cannot be considered as a statistically significant tool in the evaluation of regenerative and preneoplastic lesions in bioptically obtained specimens.
嗜银核仁组成区（AgNORs）的银染色已成为评估石蜡切片中细胞动力学以及区分良性和恶性病变的一种有价值的方法。作为区分支气管黏膜再生性改变和肿瘤前改变的一种推测性技术，我们采用银染色法，评估每个细胞核的嗜银颗粒点数及颗粒形状。通过标准化方法对每个细胞核的嗜银颗粒点数进行计数，并计算平均值和标准差。对取自活检标本的正常支气管上皮（每个细胞核1.94±0.1个颗粒，n = 12）、基底细胞增生（每个细胞核2.22±0.13个颗粒，n = 15）、杯状细胞增生（每个细胞核2.38±0.16个颗粒，n = 17）、鳞状上皮化生（每个细胞核2.58±0.17个颗粒，n = 25）、伴轻度和中度发育异常的鳞状上皮化生（每个细胞核2.50±0.5个颗粒，n = 15）、伴重度发育异常和原位癌的鳞状上皮化生（每个细胞核3.10±0.47个颗粒，n = 17）、微乳头瘤病（每个细胞核2.20±0.21个颗粒，n = 13）以及支气管早期鳞状细胞癌（每个细胞核4.40±0.74个颗粒，n = 13）进行了对比研究。在肿瘤前病变中发现嗜银颗粒的形态和大小存在差异。尽管每个细胞核的嗜银颗粒点数随增殖活性程度的增加而增多，但在评估取自活检标本的再生性和肿瘤前病变时，AgNOR方法不能被视为具有统计学意义的工具。
