Chaudhary A K, Nokubo M, Reddy G R, Yeola S N, Morrow J D, Blair I A, Marnett L J
A. B. Hancock Jr. Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232-0146.
Science. 1994 Sep 9;265(5178):1580-2. doi: 10.1126/science.8079172.
Endogenous DNA adducts may contribute to the etiology of human genetic disease and cancer. One potential source of endogenous DNA adducts is lipid peroxidation, which generates mutagenic carbonyl compounds such as malondialdehyde. A sensitive mass spectrometric method permitted detection and quantitation of the major malondialdehyde-DNA adduct, a pyrimidopurinone derived from deoxyguanosine. DNA from disease-free human liver was found to contain 5400 adducts per cell, a frequency comparable to that of adducts formed by exogenous carcinogens.
内源性DNA加合物可能在人类遗传疾病和癌症的病因学中起作用。内源性DNA加合物的一个潜在来源是脂质过氧化，其产生诱变羰基化合物，如丙二醛。一种灵敏的质谱方法能够检测和定量主要的丙二醛-DNA加合物，一种源自脱氧鸟苷的嘧啶嘌呤酮。发现来自无疾病人类肝脏的DNA每个细胞含有5400个加合物，其频率与外源性致癌物形成的加合物相当。
