利用抗毒素抗体在体外中和微囊藻毒素-LR对蛋白磷酸酶2A的抑制作用。

In vitro neutralization of the inhibitory effect of Microcystin-LR to protein phosphatase 2A by antibody against the toxin.

作者信息

Lin J R, Chu F S

机构信息

Food Research Institute, University of Wisconsin-Madison 53706.

出版信息

Toxicon. 1994 May;32(5):605-13. doi: 10.1016/0041-0101(94)90208-9.

DOI:10.1016/0041-0101(94)90208-9

PMID:8079372

Abstract

Microcystin-LR (MCYST-LR) was found to be a very potent protein-phosphatase 2A (PP2A) inhibitor at an ID50 as low as 0.1 nM. Comparing to calyculin A and okadaic acid, MCYST-LR was found to be about 100 times stronger than calyculin A and okadaic acid for their inhibition to PP2A. The inhibitory effect on PP2A by MCYST-LR was abolished when antibodies against MCYST-LR were present in the assay system. Polyclonal antibodies were more effective than monoclonal antibodies in reversing the inhibitory effect. A dose-dependent neutralization of the inhibitory effect of MCYST to PP2A by anti-MCYST polyclonal antibodies were observed. Almost 80% of the enzyme activity was restored when as low as 0.012 micrograms of Pab was present. The specific reaction caused by the antibody was evident from an analysis that the antibodies had no effect on reversing the inhibition of PP2A caused by okadaic acid and calyculin A.

摘要

微囊藻毒素-LR（MCYST-LR）被发现是一种非常有效的蛋白磷酸酶2A（PP2A）抑制剂，其半数抑制浓度（ID50）低至0.1 nM。与花萼海绵诱癌素A和冈田酸相比，MCYST-LR对PP2A的抑制作用比花萼海绵诱癌素A和冈田酸强约100倍。当检测系统中存在抗MCYST-LR抗体时，MCYST-LR对PP2A的抑制作用被消除。多克隆抗体在逆转抑制作用方面比单克隆抗体更有效。观察到抗MCYST多克隆抗体对MCYST对PP2A抑制作用的剂量依赖性中和作用。当存在低至0.012微克的多克隆抗体时，几乎80%的酶活性得以恢复。从抗体对逆转冈田酸和花萼海绵诱癌素A引起的PP2A抑制作用没有影响的分析中可以明显看出抗体引起地特异性反应。