Kamiya N, Niikura M, Ono M, Kai C, Matsuura Y, Mikami T
Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.
Virus Res. 1994 Jun;32(3):373-9. doi: 10.1016/0168-1702(94)90085-x.
Recombinant hemagglutinin-neuraminidase (rHN) and fusion (rF) glycoproteins of virulent and avirulent strains of Newcastle disease virus (NDV) expressed by using baculovirus expression system were used to investigate their protective immunization effects in chickens. The efficacy of immunization with these recombinant proteins was evaluated by challenge infection. The chickens immunized with either rHN or rF protein of a virulent strain or rHN protein of an avirulent strain were completely protected from the lethal infection of virulent NDV. On the other hand, the rF protein of an avirulent strain, in which precursor F protein was not cleaved, showed lower protective effects. Significant levels of specific antibodies against respective proteins were detected in sera from survivors, whereas relatively lower levels of antibodies were found in chickens which were killed by challenge infection. These data indicate that either HN or F protein alone could induce protective immune responses and the cleavage of F protein might be important for its immunological potential.
利用杆状病毒表达系统表达的新城疫病毒（NDV）强毒株和无毒株的重组血凝素 - 神经氨酸酶（rHN）和融合（rF）糖蛋白，用于研究它们在鸡中的保护性免疫效果。通过攻毒感染评估这些重组蛋白的免疫效力。用强毒株的rHN或rF蛋白或无毒株的rHN蛋白免疫的鸡完全受到保护，免受强毒NDV的致死性感染。另一方面，无毒株的rF蛋白（其中前体F蛋白未被切割）显示出较低的保护作用。在幸存者血清中检测到针对各自蛋白的显著水平的特异性抗体，而在因攻毒感染而死亡的鸡中发现抗体水平相对较低。这些数据表明，单独的HN或F蛋白都可以诱导保护性免疫反应，并且F蛋白的切割可能对其免疫潜力很重要。
