Vetterlein F, Prange M, Tran P N, Schmidt G
Zentrum Pharmakologie und Toxikologie, Universität, Göttingen.
Adv Exp Med Biol. 1994;345:271-3. doi: 10.1007/978-1-4615-2468-7_36.
A method is described which allows to observe the pattern of capillary plasma filling simultaneously with the redox state (NADH-fluorescence) of the myocytes in the hypoperfused myocardium. In anesthetized rats the coronary perfusion pressure was reduced to a defined level, the blood plasma labeled with dye-conjugated albumin and a myocardial biopsy-sampled. Freeze-substitution of histological sections allowed to detect the intravascular plasma label as well as the myocyte NADH-fluorescence on a microscopic level. It was found that areas showing increased cellular NADH-fluorescence did not arise in a distribution congruent with zones of failing capillary plasma flow in the hypoperfused myocardium.
本文描述了一种方法，该方法能够在观察灌注不足心肌中毛细血管血浆充盈模式的同时，观察心肌细胞的氧化还原状态（NADH荧光）。在麻醉大鼠中，将冠状动脉灌注压降至特定水平，用染料偶联白蛋白标记血浆，并取心肌活检样本。对组织切片进行冷冻置换，以便在显微镜水平上检测血管内血浆标记物以及心肌细胞NADH荧光。结果发现，在灌注不足的心肌中，细胞NADH荧光增加的区域与毛细血管血浆流动衰竭区域的分布不一致。
