同时测定麻醉大鼠灌注不足心肌中毛细血管血浆流量分布和心肌细胞氧化还原状态（NADH荧光）的方法。

Method for simultaneous determination of the distribution of capillary plasma flow and myocyte redox state (NADH-fluorescence) in the hypoperfused myocardium of the anesthetized rat.

作者信息

Vetterlein F, Prange M, Tran P N, Schmidt G

机构信息

Zentrum Pharmakologie und Toxikologie, Universität, Göttingen.

出版信息

Adv Exp Med Biol. 1994;345:271-3. doi: 10.1007/978-1-4615-2468-7_36.

DOI:10.1007/978-1-4615-2468-7_36

PMID:8079718

Abstract

A method is described which allows to observe the pattern of capillary plasma filling simultaneously with the redox state (NADH-fluorescence) of the myocytes in the hypoperfused myocardium. In anesthetized rats the coronary perfusion pressure was reduced to a defined level, the blood plasma labeled with dye-conjugated albumin and a myocardial biopsy-sampled. Freeze-substitution of histological sections allowed to detect the intravascular plasma label as well as the myocyte NADH-fluorescence on a microscopic level. It was found that areas showing increased cellular NADH-fluorescence did not arise in a distribution congruent with zones of failing capillary plasma flow in the hypoperfused myocardium.

摘要

本文描述了一种方法，该方法能够在观察灌注不足心肌中毛细血管血浆充盈模式的同时，观察心肌细胞的氧化还原状态（NADH荧光）。在麻醉大鼠中，将冠状动脉灌注压降至特定水平，用染料偶联白蛋白标记血浆，并取心肌活检样本。对组织切片进行冷冻置换，以便在显微镜水平上检测血管内血浆标记物以及心肌细胞NADH荧光。结果发现，在灌注不足的心肌中，细胞NADH荧光增加的区域与毛细血管血浆流动衰竭区域的分布不一致。