Colorado A, Brodbelt J
Department of Chemistry and Biochemistry, University of Texas, Austin 78712.
Anal Chem. 1994 Jul 15;66(14):2330-5. doi: 10.1021/ac00086a019.
A new combination of collisionally activated dissociation and ion-molecule reactions in a quadrupole ion trap mass spectrometer is developed for the class-selective determination of 4-quinolones, an important group of antibiotics. In this method, collisionally activated dissociation is used to promote dehydration of each protonated quinolone, and the resulting dehydrated fragment ion undergoes an addition reaction with an auxiliary reagent. The auxiliary reagents, including acetone, acetic anhydride, and n-propylamine, have structures that specifically allow formation of a cyclic adduct with the analyte ion. This sequential process is characteristic of a class of analyte molecules that have adjacent carboxylic acid and ketone functional groups anchored on a rigid ring and undergo facile, efficient dehydration.
在四极杆离子阱质谱仪中，开发了一种碰撞激活解离和离子-分子反应的新组合，用于对4-喹诺酮类（一类重要的抗生素）进行类别选择性测定。在该方法中，碰撞激活解离用于促进每个质子化喹诺酮的脱水，生成的脱水碎片离子与辅助试剂发生加成反应。辅助试剂包括丙酮、乙酸酐和正丙胺，其结构能特异性地与分析物离子形成环状加合物。这一连续过程是一类分析物分子的特征，这类分子具有连接在刚性环上的相邻羧酸和酮官能团，并且能轻松、高效地脱水。
