Murthy V V, Calvelli T A, Rubinstein A, Karmen A
Department of Laboratory Medicine, Albert Einstein College of Medicine, Bronx, NY 10461.
Arch Pathol Lab Med. 1994 Sep;118(9):873-7.
We report the utility of a possible lymphocyte fraction of alkaline phosphatase (ALP band-10) activity in serum to predict human immunodeficiency virus type 1 (HIV-1) infection in children born to HIV-1-seropositive mothers. The presence of ALP band 10 in serum consistently correlated with HIV-1 infection status as judged by positive HIV-1 culture, two consecutive HIV-1 p24 antigen results greater than 30 pg/mL in serum, and the subsequent confirmation of seroconversion to HIV-1 antibody after clearance of maternal IgG anti-HIV-1 antibody ascertained between 15 to 24 months post partum. Infection with HIV-1 was correctly identified in 31 samples from 18 patients ranging in age between 0.1 to 10 years; the absence of similar infection was noted in 14 samples from nine patients who served as controls and whose serum samples did not exhibit ALP band-10 activity. This ability of serum ALP band-10 activity to predict HIV-1 infection status in children as young as 2 months may be useful as a surrogate marker for early identification of HIV-1 infection in infants born to HIV-1-seropositive women long before the clearance of maternal anti-HIV-1 antibodies can be ascertained.
我们报告了血清中碱性磷酸酶可能的淋巴细胞部分（碱性磷酸酶条带10）活性在预测人类免疫缺陷病毒1型（HIV-1）感染的HIV-1血清阳性母亲所生孩子中的作用。血清中碱性磷酸酶条带10的存在与HIV-1感染状态始终相关，这一状态通过HIV-1培养阳性、血清中连续两次HIV-1 p24抗原结果大于30 pg/mL以及产后15至24个月确定母体IgG抗HIV-1抗体清除后血清转化为HIV-1抗体的后续确认来判断。在年龄介于0.1至10岁的18名患者的31份样本中正确识别出HIV-1感染；在作为对照的9名患者的14份样本中未发现类似感染，这些患者的血清样本未表现出碱性磷酸酶条带10活性。血清碱性磷酸酶条带10活性预测2个月大儿童HIV-1感染状态的这种能力，可能作为一种替代标志物，用于在能够确定母体抗HIV-1抗体清除之前很久就早期识别HIV-1血清阳性女性所生婴儿中的HIV-1感染。
