Jackel C, Krenz W D, Nagy F
Laboratoire de Neurobiologie et Physiologie Comparées, Université de Bordeaux I, Arcachon, France.
Neuroreport. 1994 May 9;5(9):1097-101. doi: 10.1097/00001756-199405000-00019.
We have characterized in crustacean neurones in culture a receptor for gamma-aminobutyric acid (GABA) which conforms to the pharmacological profile of the proposed type-C GABA receptor (GABAC) found in the vertebrate retina. It is associated with a chloride-selective ion channel and is blocked by picrotoxin. It is neither inhibited by bicuculline nor activated by baclofen, while diazepam and phenobarbital are without modulatory effect. Like the GABAC-like receptor of the vertebrate retina it is activated by the folded GABA analogue cis-4-aminocrotonic acid (CACA). Desensitization is moderate allowing for a more sustained action of GABA. Single channel recordings revealed a bicuculline-resistant GABA- and CACA-activated chloride channel with a conductance about eight times higher than that described for the bicuculline-resistant GABA receptor channel from the rat retina.
我们在培养的甲壳类神经元中鉴定出一种γ-氨基丁酸（GABA）受体，它符合在脊椎动物视网膜中发现的C型GABA受体（GABAC）的药理学特征。它与氯离子选择性离子通道相关联，并被印防己毒素阻断。它既不被荷包牡丹碱抑制，也不被巴氯芬激活，而地西泮和苯巴比妥没有调节作用。与脊椎动物视网膜的类GABAC受体一样，它被折叠的GABA类似物顺式-4-氨基巴豆酸（CACA）激活。脱敏作用适中，使得GABA能够产生更持久的作用。单通道记录显示出一种对荷包牡丹碱具有抗性的GABA和CACA激活的氯离子通道，其电导比大鼠视网膜中对荷包牡丹碱具有抗性的GABA受体通道高约八倍。
