脑室内应用脑源性神经营养因子（BDNF）和神经营养素-3（NT-3）未能保护基底核大细胞胆碱能神经元。

Intraventricular application of BDNF and NT-3 failed to protect nucleus basalis magnocellularis cholinergic neurones.

作者信息

Skup M H, Figueiredo B C, Cuello A C

机构信息

Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.

出版信息

Neuroreport. 1994 May 9;5(9):1105-9. doi: 10.1097/00001756-199405000-00021.

DOI:10.1097/00001756-199405000-00021

PMID:8080966

Abstract

Quantitative analysis of ChAT immunoreactive neurones was used to evaluate the protective potential of BDNF or NT-3 against retrograde changes in nucleus basalis magnocellularis (nbm) cholinergic neurones after unilateral partial devascularization of the rat neocortex. A daily intracerebroventricular dose of 12 micrograms, proven to be effective for NGF and aFGF in the same experimental paradigm, was administered by minipump infusion for a 1-week period. Thirty days after lesioning, neuronal shrinkage and loss of neuritic processes were not prevented by treatment. The results indicate that intracerebroventricularly delivered BDNF and NT-3 are not as effective as NGF and aFGF in protection of nbm cholinergic neurones against lesion-induced changes in adult rat brain.

摘要

采用对胆碱乙酰转移酶免疫反应性神经元进行定量分析的方法，来评估脑源性神经营养因子（BDNF）或神经营养素-3（NT-3）对大鼠新皮质单侧部分缺血后基底核大细胞部（nbm）胆碱能神经元逆行性变化的保护潜力。在相同实验范式中，已证实对神经生长因子（NGF）和成纤维细胞生长因子（aFGF）有效的每日脑室内剂量12微克，通过微型泵输注给药1周。损伤后30天，治疗未能预防神经元萎缩和神经突起的丧失。结果表明，脑室内给予BDNF和NT-3在保护成年大鼠脑内nbm胆碱能神经元免受损伤诱导的变化方面，不如NGF和aFGF有效。