Hertzler P L, Clark W H
Department of Zoology, University of California, Davis.
Zygote. 1993 Nov;1(4):287-96. doi: 10.1017/s0967199400001611.
Antibodies to sea urchin beta-tubulin and mammalian heavy neurofilaments were used to study the late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis. The neurofilament antibody fortuitously stained centrosomes in eggs, as well as the subacrosomal region and acrosomal filament in sperm. The neurofilament antibody also stained a cortical site in eggs which was associated with the positioning of the mitotic spindle. During pronuclear migration, a large maternal microtubule aster formed in addition to the sperm aster. The activity of the maternal centrosome disappeared during syngamy, while the sperm centrosomes formed the poles of the first mitotic spindle. Colcemid treatment modulated the size of the mitotic spindle and blocked pronuclear migration.
利用针对海胆β微管蛋白和哺乳动物重神经丝的抗体，研究了对虾科虾类斯氏宽虾（Sicyonia ingentis）受精的后期事件。神经丝抗体意外地对卵中的中心体以及精子中的顶体下区域和顶体丝进行了染色。神经丝抗体还对卵中的一个皮质位点进行了染色，该位点与有丝分裂纺锤体的定位有关。在原核迁移过程中，除了精子星体之外，还形成了一个大的母体微管星体。母体中心体的活性在配子融合期间消失，而精子中心体形成了第一个有丝分裂纺锤体的两极。秋水仙酰胺处理调节了有丝分裂纺锤体的大小并阻断了原核迁移。
