Shibamoto S, Hayakawa M, Takeuchi K, Hori T, Oku N, Miyazawa K, Kitamura N, Takeichi M, Ito F
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, Japan.
Cell Adhes Commun. 1994 Jan;1(4):295-305. doi: 10.3109/15419069409097261.
The effect of hepatocyte growth factor/scatter factor (HGF/SF) and epidermal growth factor (EGF) on cadherin-mediated adhesion of human carcinoma cells was studied. HGF/SF induced scattering of colonic adenocarcinoma HT29 and gastric adenocarcinomas MKN7 and MKN74 cells. Likewise, EGF induced scattering of HT29 and MKN7 cells. These cells expressed E-cadherin, which was concentrated at cell-cell contact sites. When the scattering of these cells was induced by HGF/SF or EGF, the E-cadherin concentration at cell-cell boundaries tended to decrease. Immunoblotting analyses, however, demonstrated that these growth factor treatments did not alter the expression of E-cadherin and E-cadherin-associated proteins, alpha- and beta-catenin and plakoglobin. beta-Catenin, plakoglobin and an unidentified 115-kDa molecule associated with E-cadherin were found to be phosphorylated at tyrosine residues, and these phosphorylations were enhanced by the growth factor treatments. These results suggest that HGF/SF and EGF may modulate the function of the cadherin-catenin system via tyrosine phosphorylation of cadherin-associated proteins.
研究了肝细胞生长因子/分散因子（HGF/SF）和表皮生长因子（EGF）对人癌细胞中钙黏蛋白介导的黏附作用的影响。HGF/SF诱导结肠腺癌HT29细胞以及胃腺癌MKN7和MKN74细胞发生分散。同样，EGF诱导HT29和MKN7细胞发生分散。这些细胞表达E-钙黏蛋白，其集中在细胞-细胞接触部位。当这些细胞因HGF/SF或EGF诱导而分散时，细胞-细胞边界处的E-钙黏蛋白浓度趋于降低。然而，免疫印迹分析表明，这些生长因子处理并未改变E-钙黏蛋白以及与E-钙黏蛋白相关的蛋白α-连环蛋白、β-连环蛋白和桥粒斑蛋白的表达。发现β-连环蛋白、桥粒斑蛋白以及一种与E-钙黏蛋白相关的未鉴定的115 kDa分子在酪氨酸残基处发生磷酸化，并且这些磷酸化作用因生长因子处理而增强。这些结果表明，HGF/SF和EGF可能通过钙黏蛋白相关蛋白的酪氨酸磷酸化来调节钙黏蛋白-连环蛋白系统的功能。
