Itoh Y, Johnson R, Scott B
Department of Plant Pathology, Tottori University, Japan.
Curr Genet. 1994 Jun;25(6):508-13. doi: 10.1007/BF00351670.
A high frequency transformation system has been developed for Penicillium paxilli using pAN7-1. Up to 44% of the primary transformants were heterokaryons. Loss of hygromycin resistance was observed in primary transformants that were sub-cultured on non-selective media, but single spores of these primary transformants were mitotically stable on both selective and non-selective media. A molecular analysis of the transformants generated showed that 78% had single-site integrations, with half of these containing a single copy of pAN7-1. CHEF-gel electrophoresis showed that P. paxilli has at least six chromosomes with a total genome size of about 23.4 Mb.
已利用pAN7 - 1为盘状青霉开发出一种高频转化系统。高达44%的初级转化体是异核体。在非选择性培养基上继代培养的初级转化体中观察到潮霉素抗性丧失，但这些初级转化体的单孢子在选择性和非选择性培养基上有丝分裂均稳定。对产生的转化体进行分子分析表明，78%具有单一位点整合，其中一半含有单拷贝的pAN7 - 1。正交交变电场凝胶电泳显示，盘状青霉至少有6条染色体，基因组总大小约为23.4 Mb。
