Kiuchi N, Naruse A, Yamamoto H, Sekiguchi J
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Agric Biol Chem. 1991 Dec;55(12):3053-7.
A transformation system with efficiencies between 6 and 50 stable transformants per micrograms of DNA was developed for Penicillium urticae J1 (ATCC48163) using hygromycin B-resistant plasmids containing or not containing fragments of the P. urticae genome. The tandem repeated integration and/or random integration of vector DNA were observed. Although P. urticae was unable to grow in the presence of 200 micrograms/ml hygromycin B, the transformants were resistant to more than 5 mg/ml of hygromycin B.
利用含有或不含有荨麻青霉基因组片段的潮霉素B抗性质粒，为荨麻青霉J1（ATCC48163）开发了一种转化系统，每微克DNA可产生6至50个稳定转化体。观察到载体DNA的串联重复整合和/或随机整合。尽管荨麻青霉在200微克/毫升潮霉素B存在下无法生长，但转化体对超过5毫克/毫升的潮霉素B具有抗性。
