Halfter W, Schurer B
Department of Neurobiology, University of Pittsburgh, Pennsylvania 15261.
Exp Cell Res. 1994 Sep;214(1):285-96. doi: 10.1006/excr.1994.1260.
A new heparan sulfate proteoglycan was identified by two monoclonal antibodies. The antibodies (hybridoma clones 6C4 and 1B11) were generated from mice immunized with inner limiting membranes of the embryonic chick retina. The proteoglycan had an apparent molecular weight of 250 kDa with a core protein of 180 kDa. Antibodies to perlecan and to a recently identified brain-derived heparan sulfate proteoglycan did not cross-react with purified 6C4/1B11 antigen, confirming that the three proteoglycans are not related. The 6C4/1B11 proteoglycan was abundant in basal laminae, such as the inner limiting membrane of the retina, the lens capsule, the epidermal, the pial, and the muscle basal laminae, and the vitreous body. The distribution and developmental expression of the 6C4/1B11 proteoglycan was different than that of perlecan and the brain-derived heparan sulfate proteoglycan. When used as a substrate for embryonic retinal explants, the proteoglycan did not support axonal outgrowth in vitro. The data present a new heparan sulfate proteoglycan and demonstrate the existence of at least three different heparan sulfate proteoglycans in the developing chick embryo with partially overlapping distribution.
两种单克隆抗体鉴定出一种新的硫酸乙酰肝素蛋白聚糖。这些抗体（杂交瘤克隆6C4和1B11）是由用胚胎鸡视网膜内界膜免疫的小鼠产生的。该蛋白聚糖的表观分子量为250 kDa，核心蛋白为180 kDa。针对基底膜聚糖和最近鉴定出的脑源性硫酸乙酰肝素蛋白聚糖的抗体与纯化的6C4/1B11抗原不发生交叉反应，证实这三种蛋白聚糖没有相关性。6C4/1B11蛋白聚糖在基底膜中含量丰富，如视网膜内界膜、晶状体囊、表皮、软膜和肌肉基底膜以及玻璃体。6C4/1B11蛋白聚糖的分布和发育表达与基底膜聚糖和脑源性硫酸乙酰肝素蛋白聚糖不同。当用作胚胎视网膜外植体的底物时，该蛋白聚糖在体外不支持轴突生长。这些数据展示了一种新的硫酸乙酰肝素蛋白聚糖，并证明在发育中的鸡胚中存在至少三种不同的硫酸乙酰肝素蛋白聚糖，其分布部分重叠。
