Wilson D F, Mokashi A, Chugh D, Vinogradov S, Osanai S, Lahiri S
Department of Biochemistry and Biophysics, Medical School, University of Pennsylvania, Philadelphia 19104.
FEBS Lett. 1994 Sep 12;351(3):370-4. doi: 10.1016/0014-5793(94)00887-6.
Carbon monoxide was shown to be competitive with O2 in oxygen sensing by perfused carotid bodies isolated from cats, afferent electrical activity increasing with either decreasing O2 or increasing CO. The CO-induced increase in afferent activity was fully reversed by bright light. At submaximal light intensities the extent of reversal, after correcting to equal light intensity of light quanta at each wavelength, was maximal for light of 432 +/- 2 and 590 +/- 2 nm, with a ratio (432/590) of approximately 6. This spectrum is characteristic of the CO compound of mitochondrial cytochrome a3. The photo-reversible inhibition of oxygen sensing activity by CO accounts for at least 80% of the oxygen chemosensory activity of the carotid body.
在从猫身上分离出的灌注颈动脉体的氧传感过程中，一氧化碳被证明与氧气存在竞争关系，随着氧气含量降低或一氧化碳含量增加，传入电活动增强。强光可完全逆转一氧化碳诱导的传入活动增加。在次最大光强度下，在将每个波长的光量子校正到相等光强度后，对于波长为432±2纳米和590±2纳米的光，逆转程度最大，其比例（432/590）约为6。该光谱是线粒体细胞色素a3的一氧化碳化合物的特征。一氧化碳对氧传感活性的光可逆抑制至少占颈动脉体氧化学传感活性的80%。
