Saiki K, Mogi T, Ishizuka M, Anraku Y
Department of Plant Sciences, Graduate School of Science, University of Tokyo, Japan.
FEBS Lett. 1994 Sep 12;351(3):385-8. doi: 10.1016/0014-5793(94)00889-2.
The cyoE gene of the Escherichia coli bo-type quinol oxidase operon (cyoABCDE) has been previously shown to encode heme O synthase. To demonstrate a catalytic role of a cyoE homologue (the caaE gene) in the gene cluster for caa3-type cytochrome c oxidase of thermophilic Bacillus PS3, we have carried out genetic complementation analysis using the chimeric operon cyoABCD-caaE and heme O synthase assay using the CaaE-overproduced E. coli membranes. We found that the caaE gene encodes a thermotolerant heme O synthase which provides an intermediate for heme A biosynthesis.
先前已证明大肠杆菌bo型喹啉氧化酶操纵子（cyoABCDE）的cyoE基因编码血红素O合酶。为了证明嗜热芽孢杆菌PS3的caa3型细胞色素c氧化酶基因簇中cyoE同源物（caaE基因）的催化作用，我们使用嵌合操纵子cyoABCD-caaE进行了遗传互补分析，并使用过量表达CaaE的大肠杆菌膜进行了血红素O合酶测定。我们发现caaE基因编码一种耐热的血红素O合酶，它为血红素A生物合成提供中间体。
