Piwonka E J, Firkins J L, Hull B L
Department of Dairy Science, Ohio State University, Columbus 43210.
J Dairy Sci. 1994 Jun;77(6):1570-9. doi: 10.3168/jds.S0022-0302(94)77099-5.
Three diets were fed to six cannulated heifers in a replicated 3 x 3 Latin square design. Diets were a high forage control, a high forage diet with dextrose (5.6% of DM), and a medium concentrate (39.7% of DM) diet. Diets contained 13.3% CP and 1% NaHCO3 and were fed as a TMR twice daily. Mean ruminal pH (6.47), glucose concentration (.55 mM), and reducing sugar concentration (.64 mM) in heifers were similar across diets. Rate of orchardgrass NDF digestion in situ was faster for heifers fed the dextrose than for those fed the medium concentrate diet, but both were similar to that for heifers fed the control diet. Heifers fed the medium concentrate and dextrose diets had faster ruminal particulate fractional passage rates than those fed the control diet. True ruminal and apparent total tract digestion of OM and NDF were similar among diets, but ruminal NDF digestion tended to be higher with the control than with the medium concentrate diet. Total NAN and bacterial N flow to the duodenum and efficiency of bacterial protein synthesis in the rumen were greater with the medium concentrate diet than with the control and dextrose diets. The results were consistent with others that demonstrated that factors related to nonstructural carbohydrates in the diet other than just low ruminal pH affect ruminal fiber digestion; however, these results were not as strong as those of our previous in vitro work.
采用重复的3×3拉丁方设计,给6头装有瘤胃瘘管的小母牛饲喂三种日粮。日粮分别为高粗饲料对照日粮、添加葡萄糖(占干物质的5.6%)的高粗饲料日粮和中等精料(占干物质的39.7%)日粮。日粮含有13.3%的粗蛋白和1%的碳酸氢钠,以全混合日粮的形式每日分两次饲喂。不同日粮的小母牛瘤胃平均pH值(6.47)、葡萄糖浓度(0.55 mM)和还原糖浓度(0.64 mM)相似。与饲喂中等精料日粮的小母牛相比,饲喂葡萄糖的小母牛果园草中性洗涤纤维的原位消化率更快,但二者均与饲喂对照日粮的小母牛相似。饲喂中等精料和葡萄糖日粮的小母牛瘤胃颗粒部分通过率比饲喂对照日粮的小母牛更快。不同日粮间有机物和中性洗涤纤维的真瘤胃消化率和表观全消化道消化率相似,但对照日粮的瘤胃中性洗涤纤维消化率往往高于中等精料日粮。中等精料日粮的总非氨氮和细菌氮流入十二指肠量以及瘤胃中细菌蛋白质合成效率高于对照日粮和葡萄糖日粮。这些结果与其他研究结果一致,即表明日粮中与非结构性碳水化合物有关的因素而非仅仅是低瘤胃pH值会影响瘤胃纤维消化;然而,这些结果不如我们之前的体外研究结果那么显著。