通过流式细胞术对小鼠腹腔巨噬细胞和淋巴细胞进行免疫表型分析。
Immunophenotyping of murine peritoneal macrophages and lymphocytes by flow cytometry.
作者信息
Plasman N, Vray B
机构信息
Laboratoire d'Immunologie, Faculté de Médecine, Université Libre de Bruxelles, Belgium.
出版信息
J Immunol Methods. 1994 Sep 14;174(1-2):123-31. doi: 10.1016/0022-1759(94)90016-7.
Peritoneal cells include mainly macrophages and both T and B lymphocytes. We describe a simple procedure to analyze by flow cytometry the antigens expressed on macrophage and/or lymphocyte membranes. F4/80 and CR3 macrophage antigens were detected with anti-F4/80 and anti-Mac-1 monoclonal antibodies (mAb) respectively, CD4 and THY1.2 T lymphocyte antigens with GK1.5 and J1j mAbs respectively, B CD5 lymphocytes were identified with LY1.2 mAb and Fc gamma RII/Fc gamma RIII were detected with 2.4G2 mAb.
腹膜细胞主要包括巨噬细胞以及T淋巴细胞和B淋巴细胞。我们描述了一种通过流式细胞术分析巨噬细胞和/或淋巴细胞膜上表达的抗原的简单方法。分别用抗F4/80和抗Mac-1单克隆抗体（mAb）检测F4/80和CR3巨噬细胞抗原，分别用GK1.5和J1j mAb检测CD4和THY1.2 T淋巴细胞抗原，用LY1.2 mAb鉴定B CD5淋巴细胞，并用2.4G2 mAb检测FcγRII/FcγRIII。
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