Wuyts A, Proost P, Put W, Lenaerts J P, Paemen L, van Damme J
Laboratory of Molecular Immunology, Rega Institute, University of Leuven, Belgium.
J Immunol Methods. 1994 Sep 14;174(1-2):237-47. doi: 10.1016/0022-1759(94)90028-0.
Phagocyte recruitment is an important immunological phenomenon in inflammation and cancer. A large family of selective chemotactic cytokines, designated chemokines, has recently emerged. Interleukin-8 (IL-8) is the prototype of such neutrophil activating factors, whereas MCP-1 is a well studied monocyte chemotactic protein. In vitro chemotactic assays were used to isolate and identify natural chemokines from mononuclear phagocytes and tumor cells. Additional new chemotactic proteins (MCP-2, MCP-3) attracting monocytes were also discovered by these methods. All chemokines are structurally related and show affinity for heparin. MCP-1, -2 and -3 have a comparable specific activity in monocyte chemotaxis assays. Specific and sensitive radioimmunoassays for MCP-1 and IL-8 were developed to study the regulation of their secretion by leukocytes. Monocytes or monocyte tumor cells produce MCP-1 and/or IL-8 in response to cytokines, virus, double stranded RNA, bacterial endotoxin, mitogen or phorbol ester. Granulocytes were found to secrete only minor amounts of MCP-1 and IL-8.
吞噬细胞募集是炎症和癌症中一种重要的免疫现象。最近出现了一大类选择性趋化细胞因子，即趋化因子。白细胞介素-8（IL-8）是此类中性粒细胞激活因子的原型，而单核细胞趋化蛋白-1（MCP-1）是一种经过充分研究的单核细胞趋化蛋白。体外趋化分析用于从单核吞噬细胞和肿瘤细胞中分离和鉴定天然趋化因子。通过这些方法还发现了其他吸引单核细胞的新型趋化蛋白（MCP-2、MCP-3）。所有趋化因子在结构上相关，且对肝素具有亲和力。MCP-1、-2和-3在单核细胞趋化分析中具有相当的比活性。开发了针对MCP-1和IL-8的特异性和灵敏放射免疫分析法，以研究白细胞对其分泌的调节。单核细胞或单核细胞肿瘤细胞在细胞因子、病毒、双链RNA、细菌内毒素、有丝分裂原或佛波酯的刺激下产生MCP-1和/或IL-8。发现粒细胞仅分泌少量的MCP-1和IL-8。
