Dores R M, Wasinger H, Vaudry D, Steveson T, Lancha A
Department of Biological Sciences, University of Denver, Colo 80208.
Neuroendocrinology. 1994 Jun;59(6):603-9. doi: 10.1159/000126710.
In order to determine whether Anolis carolinensis intermediate pituitary cells have the capacity to N-acetylate either ACTH(1-13)NH2 or beta-endorphin during secretion, individual intermediate pituitary explants were incubated in DMEM/CO2 for 24 h at 28 degrees C. Although alpha-melanocyte-stimulating hormone (alpha-MSH)- and beta-endorphin-related products were spontaneously released into the medium, none of these forms were N-acetylated. It appears that unlike most gnathostomes, A. carolinensis has secondarily lost the POMC-specific N-acetylation mechanisms. A ramification of this observation is that the alpha-MSH for A. carolinensis is ACTH(1-13)NH2.
为了确定卡罗来纳安乐蜥中间垂体细胞在分泌过程中是否具有将促肾上腺皮质激素（1-13）氨基末端或β-内啡肽进行N-乙酰化的能力，将单个中间垂体外植体在含二氧化碳的杜氏改良 Eagle 培养基（DMEM）中于28℃孵育24小时。尽管α-黑素细胞刺激素（α-MSH）和β-内啡肽相关产物可自发释放到培养基中，但这些形式均未被N-乙酰化。与大多数有颌类动物不同，卡罗来纳安乐蜥似乎在进化过程中失去了阿黑皮素原（POMC）特异性N-乙酰化机制。这一观察结果的一个推论是，卡罗来纳安乐蜥的α-MSH是促肾上腺皮质激素（1-13）氨基末端。
