Takei N, Ohsawa K, Imai Y, Nakao H, Iwasaki A, Kohsaka S
Department of Neurochemistry, National Institute of Neuroscience, Tokyo, Japan.
Neurosci Lett. 1994 Apr 25;171(1-2):59-62. doi: 10.1016/0304-3940(94)90604-1.
Recombinant human annexin V showed survival promoting activity in embryonic rat neocortical and mesencephalic neurons in vitro. The neurotrophic effect was observed from a relatively low dose and in a dose-dependent manner. The neurotrophic activity of annexin V was completely blocked by anti-annexin V antibody. Northern blot analysis demonstrated that annexin V mRNA is expressed in non-neuronal cells in the CNS. These results suggest that annexin V plays certain roles in the CNS as a paracrine-type neurotrophic factor.
重组人膜联蛋白V在体外对胚胎大鼠新皮质和中脑神经元具有促存活活性。从相对低剂量开始就观察到了神经营养作用，且呈剂量依赖性。膜联蛋白V的神经营养活性被抗膜联蛋白V抗体完全阻断。Northern印迹分析表明，膜联蛋白V mRNA在中枢神经系统的非神经元细胞中表达。这些结果提示，膜联蛋白V作为一种旁分泌型神经营养因子在中枢神经系统中发挥着一定作用。
