Sato M, Sugiyama T, Daimon T, Iijima K
Department of Anatomy, Akita University School of Medicine, Japan.
Neurosci Lett. 1994 Apr 25;171(1-2):97-100. doi: 10.1016/0304-3940(94)90614-9.
After sulfide-silver staining if tissue sections treated with trichloroacetic acid, silver particles indicating the copper distribution were observed in several regions of normal rat brain, including the nucleus arcuatus, nucleus tractus solitarii, nucleus habenulae medialis, locus ceruleus, and cerebellum. However, copper in these regions disappeared or was greatly decreased in LEC mutant rats. The are postrema showed no detectable level of copper in normal rat brain, but exhibited an extremely high level of copper in LEC rats, with or without the onset of jaundice. The results suggest an abnormal copper metabolism in the central nervous system of LEC rat.
用三氯乙酸处理组织切片后进行硫化银染色，在正常大鼠脑的几个区域观察到指示铜分布的银颗粒，包括弓状核、孤束核、内侧缰核、蓝斑和小脑。然而，在LEC突变大鼠中，这些区域的铜消失或大大减少。最后区在正常大鼠脑中未检测到铜水平，但在LEC大鼠中，无论有无黄疸发作，都表现出极高的铜水平。结果表明LEC大鼠中枢神经系统存在铜代谢异常。
