Gao X, Wu N, Grignon D, Zacharek A, Liu H, Salkowski A, Li G, Sakr W, Sarkar F, Porter A T
Department of Radiation Oncology (Cancer Biology Division), Wayne State University School of Medicine, Detroit, Michigan 48202.
Oncogene. 1994 Oct;9(10):2999-3003.
Mutator phenotype of nucleotide repeats has been implicated to be involved in human cancer and other diseases. This type of instability may be the direct result of DNA replication and/or repair errors. To examine mutator phenotype during the development of human prostate cancer, we undertook this study to screen 57 patients with prostatic adenocarcinoma for possible mutator phenotype at 18 microsatellite marker loci on 12 chromosomes (3p, 5q, 6p, 7p, 8p, 10q, 11p, 13q, 16q, 17p, 18q and Xq). Overall, in 37 of 57 patients, we have found positive mutator phenotype in at least one of the loci analysed. A significantly greater number of cases were found to be positive for this phenotype among the poorly differentiated than the moderately- and well-differentiated prostatic adenocarcinomas. Our data suggest that mutator phenotype may play an important role in the development and progression of human prostate cancer.
核苷酸重复序列的突变体表型被认为与人类癌症及其他疾病有关。这种类型的不稳定性可能是DNA复制和/或修复错误的直接结果。为了研究人类前列腺癌发生过程中的突变体表型，我们开展了这项研究，对57例前列腺腺癌患者进行筛查，检测12条染色体（3p、5q、6p、7p、8p、10q、11p、13q、16q、17p、18q和Xq）上18个微卫星标记位点的潜在突变体表型。总体而言，在57例患者中的37例中，我们在至少一个分析位点发现了阳性突变体表型。在低分化前列腺腺癌中，发现具有这种表型阳性的病例数显著多于中分化和高分化前列腺腺癌。我们的数据表明，突变体表型可能在人类前列腺癌的发生和发展中起重要作用。
