Bui T, Faltynek C R, Ho R J
Department of Pharmaceutics, University of Washington School of Pharmacy, Seattle 98195.
Vaccine. 1994 May;12(7):646-52. doi: 10.1016/0264-410x(94)90271-2.
We investigated the biological activity of human recombinant interleukin-7 (rhIL-7) on guinea-pig cells in vitro and in vivo. RhIL-7 can stimulate plastic non-adherent cells of guinea-pigs to proliferate in vitro at a degree comparable to that of human cells. Administration of rhIL-7 to guinea-pigs significantly increased their white blood cell concentration, specifically the lymphocyte population. The effect of rhIL-7 on circulating blood cells was dose dependent, in that 14 doses of twice daily subcutaneous injections at 8 x 10(5) U kg-1 and 2.4 x 10(6) U kg-1 rhIL-7 increased peripheral blood lymphocyte concentrations by 38% (p = 0.047) and 139% (p = 0.0005), respectively, compared with the placebo group. Two weekly doses of rhIL-7 liposome (5.6 x 10(6) U kg-1 dose, equivalent to 11.2 x 10(6) U kg-1 total dose) elicited a similar effect on the lymphocyte population, comparable to that of the twice daily administrations of 8 x 10(5) U kg-1 soluble rhIL-7 for 7 days (7 days x two doses/day x 8 x 10(5) U kg-1 = 11.2 x 10(6) U kg-1 total dose). However, the increased lymphocyte count induced by rhIL-7 administration did not protect guinea-pigs from the primary herpes simplex virus (HSV)-2 infection. Only when rhIL-7 was given in association with HSV-antigen gD in an antigen-specific mode did it exhibit an enhanced protective effect against the sublethal dose of genital HSV-2 challenge.(ABSTRACT TRUNCATED AT 250 WORDS)
我们在体外和体内研究了重组人白细胞介素-7（rhIL-7）对豚鼠细胞的生物学活性。rhIL-7能够刺激豚鼠的塑料非贴壁细胞在体外增殖，增殖程度与人细胞相当。给豚鼠注射rhIL-7可显著提高其白细胞浓度，尤其是淋巴细胞数量。rhIL-7对循环血细胞的作用呈剂量依赖性，即每天皮下注射2次，连续注射14天，剂量分别为8×10⁵U/kg和2.4×10⁶U/kg的rhIL-7，与安慰剂组相比，外周血淋巴细胞浓度分别增加了38%（p = 0.047）和139%（p = 0.0005）。每周注射2次rhIL-7脂质体（剂量为5.6×10⁶U/kg，相当于总剂量11.2×10⁶U/kg）对淋巴细胞数量产生了类似的影响，与每天2次注射8×10⁵U/kg可溶性rhIL-7，连续注射7天（7天×每天2次×8×10⁵U/kg = 11.2×10⁶U/kg总剂量）的效果相当。然而，注射rhIL-7所诱导的淋巴细胞计数增加并不能保护豚鼠免受原发性单纯疱疹病毒2型（HSV-2）感染。只有当rhIL-7以抗原特异性方式与HSV抗原gD联合使用时，才对亚致死剂量的生殖器HSV-2攻击表现出增强的保护作用。（摘要截短于250字）
