抗马软骨细胞、成骨细胞和骨细胞单克隆抗体的制备与鉴定

Preparation and characterization of monoclonal antibodies against equine chondrocytes, osteoblasts and osteocytes.

作者信息

Katayama Y, Oikawa M, Kaneko M, Yoshihara T, Yoshikawa H, Yoshikawa T

机构信息

Pathology Division, Equine Research Institute, Japan Racing Association, Tokyo.

出版信息

Zentralbl Veterinarmed A. 1994 Feb;41(1):31-6. doi: 10.1111/j.1439-0442.1994.tb00062.x.

DOI:10.1111/j.1439-0442.1994.tb00062.x

PMID:8085396

Abstract

Three monoclonal antibodies capable of individually recognizing chondrocytes, osteoblasts and osteocytes were prepared. EB-1 reacted with a 55-kDa antigen on the chondrocyte membrane, EB-2 with a 110-kDa antigen on the membrane of osteoblasts and/or partial osteocytes, and EB-3 with a 130-kDa antigen on the membrane of osteocytes. These monoclonal antibodies may be useful probes for studying the differentiation and maturation of osteogenic cells.

摘要

制备了三种能够分别识别软骨细胞、成骨细胞和骨细胞的单克隆抗体。EB-1与软骨细胞膜上一种55 kDa的抗原发生反应，EB-2与成骨细胞和/或部分骨细胞膜上一种110 kDa的抗原发生反应，EB-3与骨细胞膜上一种130 kDa的抗原发生反应。这些单克隆抗体可能是研究成骨细胞分化和成熟的有用探针。