Yasunaga M, Nishikawa S
Dept. of Molecular Genetics Faculty of Medicine, Kyoto University, Japan.
Gan To Kagaku Ryoho. 1994 Sep;21(12):2077-83.
IL-7, a 25-kDa glycoprotein was cloned as a cytokine derived from bone marrow stromal cells, which induces proliferation of pre-B cells. IL-7 was found to be produced by the thymus, spleen, kidney and bone marrow stromal cells and induces proliferation of immature B and T cells. It has no effects on mature B cells but induce proliferation of mature T cells IL-7 R is expressed during the stages; B 220+ sIgM- of pre-B cells but not sIgM+ mature B cells, and also expressed CD4-8-, CD4+8- CD4-8+ T cells. It is shown that IL-7 can act only on these IL7R+ cells functionally in vitro and in vivo. We and other group demonstrated that IL-7, when used in combination with stromal cells or SCF can induce proliferation of pre-B cells. Recently we generated successfully SCF and IL-7 dependent pre-B cell lines, and now have used them for cell cycle analysis. To date, it was found that IL-7 is required for G1 to S transition. IL-7 also acts as a co-stimulus for the proliferation of antigen- or mitogen-activated T cells and can induce CTL response and LAK activity.
白细胞介素-7（IL-7）是一种25千道尔顿的糖蛋白，作为一种源自骨髓基质细胞的细胞因子被克隆出来，它可诱导前B细胞增殖。人们发现IL-7由胸腺、脾脏、肾脏和骨髓基质细胞产生，可诱导未成熟B细胞和T细胞增殖。它对成熟B细胞无作用，但可诱导成熟T细胞增殖。IL-7受体在以下阶段表达：前B细胞的B220+sIgM-阶段，而非sIgM+成熟B细胞阶段，并且在CD4-8-、CD4+8-、CD4-8+T细胞中也有表达。研究表明，IL-7在体外和体内仅能对这些IL-7R+细胞发挥功能作用。我们和其他研究小组证明，IL-7与基质细胞或干细胞因子（SCF）联合使用时，可诱导前B细胞增殖。最近我们成功建立了依赖SCF和IL-7的前B细胞系，现在已将它们用于细胞周期分析。迄今为止，已发现G1期向S期转变需要IL-7。IL-7还作为抗原或丝裂原激活的T细胞增殖的共刺激因子，可诱导细胞毒性T淋巴细胞（CTL）反应和淋巴因子激活的杀伤细胞（LAK）活性。
