Rodella L, Rezzani R, Corsetti G, Stacchiotti A, Ventura R G
Department of Biomedical Sciences and Biotechnology, University of Brescia.
Boll Soc Ital Biol Sper. 1994 Apr;70(4):69-74.
The number and proportion of motoneurons and interneurons present in the rat abducens nucleus was determined by the use of ChAT immunostaining and of HRP staining after retrograde transport from the injected right lateral rectus muscle. After HRP injection 67% of abducens neurons took up HRP and were hence motoneurons to the muscle. The cell bodies were mainly located in the middle third of the nucleus and were either spindle-shaped or pyriform. By ChAT-immunohistochemistry, 77% of the rat abducens neurons were ChAT-positive. After considering and discarding the hypotheses that the lateral rectus muscle could be incompletely filled by HRP, and that other muscles may be innervated by abducens motoneurons, it is concluded that some interneurons of the abducens nucleus of the rat are probably cholinergic.
通过使用胆碱乙酰转移酶（ChAT）免疫染色以及从注射的右侧外直肌逆行运输后的辣根过氧化物酶（HRP）染色，确定了大鼠展神经核中运动神经元和中间神经元的数量及比例。注射HRP后，67%的展神经神经元摄取了HRP，因此是该肌肉的运动神经元。细胞体主要位于核的中三分之一处，呈纺锤形或梨形。通过ChAT免疫组织化学方法，77%的大鼠展神经神经元ChAT呈阳性。在考虑并排除了外直肌可能未被HRP完全填充以及其他肌肉可能由展神经运动神经元支配的假设后，得出结论：大鼠展神经核的一些中间神经元可能是胆碱能的。
