Lu P W, Briody J N, Howman-Giles R, Trube A, Cowell C T
Robert Vines Growth Research Centre, Royal Alexandra Hospital for Children, Sydney, Australia.
Bone. 1994 Mar-Apr;15(2):199-202. doi: 10.1016/8756-3282(94)90708-0.
The present study evaluated the use of a small animal total body software of dual energy x-ray absorptiometry (DXA) in the assessment of total body and regional bone mineral content (BMC) and bone mineral density (BMD) in small rats. Twenty-three rats, with weights ranging from 146 to 246 g, were included in the study. All were scanned using the same software version and same scan procedure (speed and scanned area). Total body BMD, BMC, and body weight were measured by DXA in each rat. Femoral BMC and BMD were analyzed by using regional analysis facilities. The repeatability (precision) of this software version was assessed prior to the study and the coefficients of variation (CV) were 2.9% for total body BMC, 0.8% for total body BMD, 1.2% for body weight, and 2.2% for mean femoral BMD. DXA measurements were compared with the measurements obtained by using established standards, namely weight and bone ash content. Total body ash content and femoral ash content were measured separately in all rats. There was a strong linear correlation between BMC and ash content in total body (r2 = 0.98, p = 0.0001) and in femur (r2 = 0.94, p = 0.0001). There was also an excellent linear association between body weight measured by DXA and scale weight (r2 = 0.99, p = 0.0001). We conclude that this software version is suitable for study on small animals and is a useful tool for assessment of regional as well as total body bone mineral status.
本研究评估了一种小动物全身双能X线吸收测定法（DXA）软件在评估小型大鼠全身和局部骨矿物质含量（BMC）及骨矿物质密度（BMD）方面的应用。23只体重在146至246克之间的大鼠被纳入研究。所有大鼠均使用相同的软件版本和相同的扫描程序（速度和扫描区域）进行扫描。通过DXA测量每只大鼠的全身BMD、BMC和体重。使用局部分析功能分析股骨的BMC和BMD。在研究之前评估了该软件版本的重复性（精密度），全身BMC的变异系数（CV）为2.9%，全身BMD为0.8%，体重为1.2%，股骨平均BMD为2.2%。将DXA测量结果与使用既定标准（即体重和骨灰含量）获得的测量结果进行比较。分别测量所有大鼠的全身骨灰含量和股骨骨灰含量。全身BMC与骨灰含量之间（r2 = 0.98，p = 0.0001）以及股骨中（r2 = 0.94，p = 0.0001）存在强线性相关性。DXA测量的体重与秤重之间也存在极好的线性关联（r2 = 0.99，p = 0.0001）。我们得出结论，该软件版本适用于小动物研究，是评估局部以及全身骨矿物质状态的有用工具。
