Sims D E, Horne M M
Department of Anatomy and Physiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
Eur J Morphol. 1994 Mar;32(1):59-64.
A fixative composed of 1% w/v osmium tetroxide dissolved in a tluorocarbon (FC-72) was used to perfusion-fix rat blood vessels for this ultrastructural study. With non-aqueous fixation, the lumenal surfaces of endothelial cells in cardiac and skeletal muscles retained an extracellular coat which was morphologically consistent with glycocalyx. Venules displayed the greatest density and depth (up to 870 nm thick) of glycocalyx, but all vessel types had at least 70 nm of surface coat. The results indicate that non-aqueous fixatives will be useful in studies of the endothelial cell lumenal surface, while offering excellent preservation of general ultrastructure.
在这项超微结构研究中，使用了一种由溶解于氟碳化合物（FC - 72）中的1%（重量/体积）四氧化锇组成的固定剂对大鼠血管进行灌注固定。通过非水固定，心脏和骨骼肌中内皮细胞的管腔表面保留了一层细胞外被，其形态与糖萼一致。微静脉显示出糖萼的最大密度和深度（厚达870纳米），但所有血管类型的表面被膜至少有70纳米厚。结果表明，非水固定剂将有助于内皮细胞管腔表面的研究，同时能出色地保存一般超微结构。
