Hu X, Emanuel P D, Zuckerman K S
Division of Medical Oncology and Hematology, University of South Florida, Tampa 33612.
Biochim Biophys Acta. 1994 Sep 8;1223(2):306-8. doi: 10.1016/0167-4889(94)90241-0.
Two distinct cDNA clones, corresponding to alternative mRNA splicing variants of the alpha subunit of the granulocyte-macrophage colony-stimulating factor receptor (GM-CSF-R alpha) were isolated from human blood mononuclear cells by RT-PCR. The first one lacks 179 bp, and the second one lacks 136 bp of the major variant, GM-CSF-R alpha 1. Both of variants result in shifts in the reading frame and are expected to encode 377- and 285-amino-acid membrane anchoring and soluble receptor isoforms, respectively.
通过逆转录聚合酶链反应（RT-PCR）从人血单核细胞中分离出两个不同的互补DNA（cDNA）克隆，它们对应于粒细胞-巨噬细胞集落刺激因子受体（GM-CSF-Rα）α亚基的可变信使核糖核酸（mRNA）剪接变体。第一个变体缺少179碱基对（bp），第二个变体缺少主要变体GM-CSF-Rα1的136 bp。这两个变体均导致阅读框移位，预计分别编码377个和285个氨基酸的膜锚定和可溶性受体同工型。
