Alves A M, De Almeida D F, von Krüger W M
Laboratório de Fisiologia Celular, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
J Eukaryot Microbiol. 1994 Jul-Aug;41(4):415-9. doi: 10.1111/j.1550-7408.1994.tb06099.x.
Reversible changes in kinetoplast DNA (kDNA) minicircles sequences were observed in clones of Trypanosoma cruzi strain Y, following a number of passages during exponential growth phase or after subcloning in blood-free medium. kDNA restriction patterns of clones were similar to those of the original uncloned strain, while subclones presented distinct kDNA restriction patterns. Homology experiments demonstrated strong hybridization between kDNA with the same electrophoretic mobility patterns while only weak signals were observed with kDNA of different patterns. The changes observed, which are unprecedented in T. cruzi clones, characterize transkinetoplastidy, and seem to be associated with similarly reversible changes both in zymodeme and in infectivity.
在克氏锥虫Y株的克隆中观察到动基体DNA（kDNA）微小环序列的可逆变化，这些变化发生在指数生长期的多次传代后或在无血培养基中进行亚克隆后。克隆的kDNA限制性图谱与原始未克隆菌株的图谱相似，而亚克隆呈现出不同的kDNA限制性图谱。同源性实验表明，具有相同电泳迁移模式的kDNA之间存在强杂交信号，而不同模式的kDNA之间仅观察到微弱信号。观察到的这些变化在克氏锥虫克隆中是前所未有的，其特征为转动基体现象，并且似乎与酶谱和感染性方面类似的可逆变化相关。
