Camandaroba Edson L P, Reis Eliana A G, Reis Mitermayer G, Andrade Sonia G
Laboratorio de Histologia e Embriologia Animal Comparada, Universidade Estadual de Feira de Santana, Feira de Santana, BA, Brazil.
Mem Inst Oswaldo Cruz. 2006 Sep;101(6):613-5. doi: 10.1590/s0074-02762006000600005.
Colombian strain of Trypanosoma cruzi, biodeme Type III (T. cruzi I), has been cloned by micromanipulation at two phases of the acute infection: early (10 days ) and advanced (30 days). Twelve clones were obtained therefrom. Characterization by their biological and biochemical behavior showed an identity among the several clones and their parental strain, albeit with different degrees of virulence. Molecular characterization of the kinetoplast DNA (kDNA) after amplification by polymerase chain reaction revealed identical profiles of the bands from the kDNA minicircle by the analysis of restriction fragment length polymorphism for the isolated clones, their parental strain, and to the clones isolated at two different phases of the infection. Results suggest the predominance of a "principal clone", in the composition of the Colombian strain, responsible for the biological and biochemical behavior. However, no relationship was detected between the molecular profile of kDNA and the degree of virulence presented by the several clones.
克氏锥虫的哥伦比亚菌株，生物型III（克氏锥虫I），已在急性感染的两个阶段通过显微操作进行克隆：早期（10天）和晚期（30天）。从中获得了12个克隆。通过其生物学和生化行为进行的表征显示，几个克隆与其亲本菌株之间具有一致性，尽管毒力程度不同。通过聚合酶链反应扩增后对动质体DNA（kDNA）进行分子表征，通过对分离克隆、其亲本菌株以及在感染的两个不同阶段分离的克隆进行限制性片段长度多态性分析，揭示了来自kDNA小环的条带具有相同的图谱。结果表明，在哥伦比亚菌株的组成中，存在一个“主要克隆”，它决定了生物学和生化行为。然而，未检测到kDNA的分子图谱与几个克隆所呈现的毒力程度之间存在关联。
