Mikulecky D C, Thellier M
Department of Physiology, Medical College of Virginia Commonwealth University, Richmond 23298-0551.
C R Acad Sci III. 1993 Dec;316(12):1399-403.
As an example of the application of network thermodynamics to the treatment of complicated enzymatic systems, we have studied the transient kinetic behavior of a sequence of five enzymatic reactions, four with Michaelis-Menten kinetics and the final one with sigmoid kinetics. The object was to determine how the time-courses of the concentrations of all the intermediate substrates involved, depend on the effect of forward activation by the first substrate on the final enzymatic step. The case with forward activation exhibited an unexpected behavior with a reversal of the direction of the reaction before reaching equilibrium. The solution of the set of five non-linear differential equations was achieved using the student version of the simulation package PSPICE. The same approach can be utilized to study the behavior of any type of complex multienzymatic system (steady-states, transients, oscillations, chaos), or of combinations of enzymatic reactions with transmembrane transport in compartmental systems.
作为网络热力学在复杂酶系统治疗中应用的一个例子，我们研究了一系列五个酶促反应的瞬态动力学行为，其中四个具有米氏动力学，最后一个具有S型动力学。目的是确定所有涉及的中间底物浓度的时间进程如何取决于第一种底物对最终酶促步骤的正向激活作用。正向激活的情况表现出意想不到的行为，即在达到平衡之前反应方向发生逆转。使用模拟软件包PSPICE的学生版实现了这组五个非线性微分方程的求解。同样的方法可用于研究任何类型的复杂多酶系统（稳态、瞬态、振荡、混沌），或酶促反应与隔室系统中跨膜运输的组合的行为。
