无胸腺小鼠脾细胞中未成熟T细胞因铜绿假单胞菌外毒素A而增殖。

Proliferation of immature T cells within the splenocytes of athymic mice by Pseudomonas exotoxin A.

作者信息

Dixon D M, Misfeldt M L

机构信息

Department of Molecular Microbiology and Immunology, University of Missouri-Columbia, School of Medicine 65212.

出版信息

Cell Immunol. 1994 Oct 1;158(1):71-82. doi: 10.1006/cimm.1994.1257.

DOI:10.1006/cimm.1994.1257

PMID:8087869

Abstract

Pseudomonas aeruginosa exotoxin A has been shown to stimulate splenocytes from athymic nude mice. The present studies were performed to characterize the exotoxin A-responsive cells within the splenocytes of athymic nude mice. The results of these studies indicate that exotoxin A-responsive cells were represented in the nylon wool-adherent cell population and in the Ig-depleted splenocyte population (IgNA). Flow microfluorimetry analysis indicated that exotoxin A induced the expansion of Thy1+, CD3-, CD4-, and CD8- cells which also expressed the heat-stable antigen (HSA), an antigen expressed on immature T lymphocytes. Depletion of Thy1+ cells abrogated the exotoxin A-induced proliferation; however, depletion of CD4+ and CD8+ cells did not affect the exotoxin A-induced proliferation of IgNA cells. Thus, the results indicate that exotoxin A stimulates the proliferation of immature T cells within the splenocytes of nude mice that are Thy1+, HSA+, CD3-, CD4-, CD8-.

摘要

铜绿假单胞菌外毒素A已被证明可刺激无胸腺裸鼠的脾细胞。本研究旨在鉴定无胸腺裸鼠脾细胞中对外毒素A有反应的细胞。这些研究结果表明，对外毒素A有反应的细胞存在于尼龙毛黏附细胞群体和Ig耗尽的脾细胞群体（IgNA）中。流式微量荧光测定分析表明，外毒素A可诱导Thy1 +、CD3 -、CD4 -和CD8 -细胞扩增，这些细胞还表达热稳定抗原（HSA），这是一种在未成熟T淋巴细胞上表达的抗原。Thy1 +细胞的耗尽消除了外毒素A诱导的增殖；然而，CD4 +和CD8 +细胞的耗尽并不影响外毒素A诱导的IgNA细胞增殖。因此，结果表明外毒素A可刺激裸鼠脾细胞内Thy1 +、HSA +、CD3 -、CD4 -、CD8 -的未成熟T细胞增殖。