粗糙脉孢菌中一个编码泛素-40S核糖体蛋白融合蛋白的基因（ubi::crp-6）的分离与鉴定。

The isolation and characterization of a Neurospora crassa gene (ubi::crp-6) encoding a ubiquitin-40S ribosomal protein fusion protein.

作者信息

Tarawneh K A, Anumula K R, Free S J

机构信息

Department of Biological Sciences, State University of New York at Buffalo 14260.

出版信息

Gene. 1994 Sep 15;147(1):137-40. doi: 10.1016/0378-1119(94)90053-1.

DOI:10.1016/0378-1119(94)90053-1

PMID:8088539

Abstract

We have isolated and sequenced a Neurospora crassa gene encoding a single copy of ubiquitin (UBI) fused to the S27a ribosomal (r) protein. We have opted to name this gene the ubiquitin/cytoplasmic r-protein gene 6 (ubi::crp-6). The ubi::crp-6 gene generates a 700-nucleotide (nt) transcript. It shares a 700-bp regulatory region with the cytoplasmic r-protein gene 5 (crp-5), a gene encoding the N. crassa S26 r-protein. The two genes are transcribed divergently from the common regulatory region and their mRNA levels are regulated in parallel during growth on a variety of carbon sources.

摘要

我们已经分离并测序了粗糙脉孢菌中一个编码与S27a核糖体（r）蛋白融合的单拷贝泛素（UBI）的基因。我们选择将该基因命名为泛素/细胞质r蛋白基因6（ubi::crp-6）。ubi::crp-6基因产生一个700个核苷酸（nt）的转录本。它与细胞质r蛋白基因5（crp-5）共享一个700碱基对（bp）的调控区域，crp-5基因编码粗糙脉孢菌的S26 r蛋白。这两个基因从共同的调控区域反向转录，并且在多种碳源上生长期间，它们的mRNA水平受到平行调控。