Knight J C, Grimaldi G, Thiesen H J, Bech-Hansen N T, Fletcher C D, Coleman M P
Department of Histopathology, St. Thomas's Hospital, UMDS, London, United Kingdom.
Genomics. 1994 May 1;21(1):180-7. doi: 10.1006/geno.1994.1240.
The ZNF21, ZNF41, and ZNF81 genes encode Krüppel-type zinc-finger proteins (ZFPs) and have previously been mapped to chromosome Xp. Published data describing the clustering of ZFP genes on human autosomes led us to investigate the organization of ZNF21, ZNF41, and ZNF81 on the X chromosome. Rodent-human hybrid analysis sublocalized all three genes to Xp22.11-p11.23. ZNF21, ZNF41, and ZNF81 were then shown to segregate within a series of YACs (95 to 730 kb) containing known markers at Xp11.23, such that these YACs could be assembled into a contig spanning approximately 1.5 Mb of DNA. Southern analysis of intact YACs and YAC DNAs cut with rare-cutter restriction enzymes enabled us to establish the spatial organization of the ZFP gene cluster, the OATL1 pseudogene, the recurrent t(X;18) chromosome translocation breakpoint in synovial sarcoma, and the previously described cluster of ARAF1, SYN1, TIMP, and PFC genes. We have assigned the ETS-related gene ELK1 to a locus tightly linked to the PFC gene; the entire cluster of five genes is contained within a distance of 120 kb. ZNF41 maps to a 440-kb YAC spanning this region, while a more proximal cluster comprising the ZNF21 and ZNF81 genes lies 150 kb distal to the chromosome breakpoint associated with synovial sarcoma.
ZNF21、ZNF41和ZNF81基因编码Krüppel型锌指蛋白（ZFP），此前已被定位到X染色体短臂。已发表的关于人类常染色体上ZFP基因聚类的数据促使我们研究ZNF21、ZNF41和ZNF81在X染色体上的组织情况。啮齿动物-人类杂交分析将这三个基因都亚定位到Xp22.11-p11.23。随后发现ZNF21、ZNF41和ZNF81在一系列包含Xp11.23已知标记的酵母人工染色体（YAC，95至730 kb）中分离，这样这些YAC就可以组装成一个跨越约1.5 Mb DNA的重叠群。对完整YAC和用稀有切割限制酶切割的YAC DNA进行Southern分析，使我们能够确定ZFP基因簇、OATL1假基因、滑膜肉瘤中复发性t(X;18)染色体易位断点以及先前描述的ARAF1、SYN1、TIMP和PFC基因簇的空间组织。我们已将ETS相关基因ELK1定位到与PFC基因紧密连锁的一个位点；这五个基因的整个簇位于120 kb的距离内。ZNF41定位到一个跨越该区域的440-kb YAC，而包含ZNF21和ZNF81基因的更近端簇位于与滑膜肉瘤相关的染色体断点远端150 kb处。
