Legerski R J, Liu P, Li L, Peterson C A, Zhao Y, Leach R J, Naylor S L, Siciliano M J
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Genomics. 1994 May 1;21(1):266-9. doi: 10.1006/geno.1994.1256.
The human gene XPC (formerly designated XPCC), which corrects the repair deficiency of xeroderma pigmentosum (XP) group C cells, was mapped to 3p25. A cDNA probe for Southern blot hybridization and diagnostic PCR analyses of hybrid clone panels informative for human chromosomes in general and portions of chromosome 3 in particular produced the initial results. Fluorescence in situ hybridization utilizing both a yeast artificial chromosome DNA containing the gene and XPC cDNA as probes provided verification and specific regional assignment. A conflicting assignment of XPC to chromosome 5 is discussed in light of inadequacies in the exclusive use of microcell-mediated chromosome transfer for gene mapping.
