Sarker A B, Koirala T R, Jeon H J, Murakami I
Department of Pathology, Okayama University Medical School, Japan.
Indian J Pathol Microbiol. 1994 Jan;37(1):29-38.
Normal bronchopulmonary tissues and pulmonary carcinomas including three major types (squamous cell carcinoma, adenocarcinoma, and small-cell carcinoma) were studied using three biotinylated lectins (Bauhinia purpurea [BPA], Phaseolus vulgaris [PHA], and Maclura pomifera [MPA]) by avidin biotin peroxidase complex (ABC) method. The study demonstrated that BPA binds with macrophages and pneumocytes of normal tissue, and with adenocarcinoma and small-cell carcinoma, but nonreactive with squamous cell carcinoma. PHA and MPA bound to all the normal components of bronchopulmonary tree and carcinomas of all types. Adenocarcinoma showed the highest density of reacting sites for BPA and MPA, and squamous cell carcinoma showed the highest binding sites for PHA, while small-cell carcinoma were the lowest reacting variant for all lectins. Lectins used in this study have limited usefulness for the diagnosis of pulmonary neoplasms.
采用抗生物素蛋白-生物素过氧化物酶复合物（ABC）法，使用三种生物素化凝集素（紫羊蹄甲[BPA]、菜豆[PHA]和桑科柘属植物[MPA]）对正常支气管肺组织及包括三种主要类型（鳞状细胞癌、腺癌和小细胞癌）的肺癌进行了研究。研究表明，BPA与正常组织的巨噬细胞和肺细胞结合，也与腺癌和小细胞癌结合，但与鳞状细胞癌无反应。PHA和MPA与支气管肺树的所有正常成分及所有类型的癌结合。腺癌对BPA和MPA的反应位点密度最高，鳞状细胞癌对PHA的结合位点最高，而小细胞癌对所有凝集素的反应性变体最低。本研究中使用的凝集素对肺肿瘤的诊断作用有限。
