Landers J P, Subramaniam M, Gosse B, Weinshilboum R, Madden B J, Spelsberg T C
Department of Biochemistry and Molecular Biology, Mayo Clinic/Foundation, Rochester, Minnesota 55905.
J Cell Biochem. 1994 Jun;55(2):241-51. doi: 10.1002/jcb.240550211.
The avian oviduct receptor binding factor-1 (RBF-1) is a 10 kDa nuclear matrix protein that was originally identified through its ability to effect high affinity interaction of activated progesterone receptor (PR) with chromatin. In the present study, the RBF-1 is shown to not be restricted to reproductive tissues (e.g., oviduct) but present in all avian tissues examined by Western blot analysis with a monoclonal antibody prepared against purified RBF-1. The heart and pancreas had the highest and lowest RBF-1 levels, respectively; the concentration ranging by approximately 50-fold in these tissues. The 10 kDa size of the RBF-1 detected in all tissues suggests no significant tissue-specific differences in the protein. This was consistent with the finding that purified hepatic and oviductal RBF-1 have identical amino-terminal sequence. Using a recently isolated cDNA to RBF-1, the levels of RBF-1 mRNA were found to correlate well with the ubiquitous presence of the protein as well as tissue-specific differences in concentration. The presence of RBF-1 in non-progesterone responsive tissues suggests the possibility that RBF-1 may not be specifically involved in PR-DNA interactions but may play a more diverse role, possibly involving other steroid receptors such as the glucocorticoid receptor.
禽输卵管受体结合因子-1（RBF-1）是一种10 kDa的核基质蛋白，最初是通过其影响活化的孕激素受体（PR）与染色质的高亲和力相互作用的能力而被鉴定出来的。在本研究中，通过用针对纯化的RBF-1制备的单克隆抗体进行蛋白质免疫印迹分析表明，RBF-1并不局限于生殖组织（如输卵管），而是存在于所有检测的禽类组织中。心脏和胰腺的RBF-1水平分别最高和最低；这些组织中的浓度范围约为50倍。在所有组织中检测到的10 kDa大小的RBF-1表明该蛋白质没有明显的组织特异性差异。这与纯化的肝脏和输卵管RBF-1具有相同的氨基末端序列这一发现一致。使用最近分离的RBF-1 cDNA，发现RBF-1 mRNA水平与该蛋白质的普遍存在以及浓度的组织特异性差异密切相关。RBF-1在非孕激素反应性组织中的存在表明，RBF-1可能不专门参与PR-DNA相互作用，而是可能发挥更广泛的作用，可能涉及其他类固醇受体，如糖皮质激素受体。
