叶酸与血清蛋白的结合。V. 肝脏中的氚化叶酸。

Binding of folic acid to serum proteins. V. Tritiated folic acid in the liver.

作者信息

Markkanen T, Pajula R L, Virtanen S, Himanen P, Järvinen M

出版信息

Acta Haematol. 1975;53(6):329-35. doi: 10.1159/000208201.

DOI:10.1159/000208201

Abstract

Sephadex G-200 chromatographies of the liver cell sap of rabbits, 1, 3 and 6 days after an injection of 3H-folic acid activity (FAA) into the circulation, were used in an attempt to study the binding of this labelled FAA to liver proteins. The labelled FAA was quickly accumulated in the liver, and in the cell sap it was grouped chromatographically to two maxima, which corresponded to the two maxima of microbiological FAA (L. casei). The maxima were eluated in the chromatography together with cell sap proteins. The possible coupling of FAA to proteins in the liver is discussed.

摘要

对家兔在向循环系统注射³H-叶酸活性物质（FAA）后1天、3天和6天的肝细胞液进行葡聚糖凝胶G - 200色谱分析，以尝试研究这种标记的FAA与肝脏蛋白质的结合情况。标记的FAA迅速在肝脏中积累，在细胞液中通过色谱分析可分为两个峰，这与微生物学方法测定的FAA（干酪乳杆菌）的两个峰相对应。这些峰在色谱分析中与细胞液蛋白质一起被洗脱出来。文中讨论了FAA与肝脏中蛋白质可能的结合情况。