Scott P M, Delgado T, Prelusky D B, Trenholm H L, Miller J D
Food Research Division, Bureau of Chemical Safety, Food Directorate, Ottawa, Ontario, Canada.
J Environ Sci Health B. 1994 Sep;29(5):989-98. doi: 10.1080/03601239409372913.
Fumonisin B1 (FB1) and fumonisin B2 (FB2) were determined in milk by liquid chromatography (LC) following immunoaffinity column cleanup. Recoveries from milk spiked with 5-50 ng each fumonisin/ml averaged 79-109%. The aminopentol hydrolysis product of FB1 (AP1) was determined by LC after cleanup on a C18 solid phase phase extraction column; mean recoveries were 69-83% at spiking levels of 50-100 ng AP1/ml milk. Detection limits were of the order 3-7 ng/ml for FB1 and FB2, and 20-25 ng/ml for AP1. A stability study showed no losses of FB1 and FB2 in milk under conditions of freezing, refrigeration and boiling. A transmission study using four cows dosed with pure FB1 either orally (1.0 and 5.0 mg FB1/kg b.w.) or by i.v. injection (0.05 and 0.20 mg FB1/kg b.w.) showed no detectable residues of FB1 or AP1 in the milk, with or without hydrolytic treatment with beta-glucuronidase/sulfatase to liberate any conjugates.
采用免疫亲和柱净化后，通过液相色谱法（LC）测定牛奶中的伏马毒素B1（FB1）和伏马毒素B2（FB2）。向牛奶中添加浓度为5 - 50 ng/ml的各伏马毒素，回收率平均为79 - 109%。FB1的氨基戊醇水解产物（AP1）在C18固相萃取柱净化后，通过LC测定；在牛奶中添加浓度为50 - 100 ng AP1/ml时，平均回收率为69 - 83%。FB1和FB2的检测限约为3 - 7 ng/ml，AP1的检测限为20 - 25 ng/ml。稳定性研究表明，在冷冻、冷藏和煮沸条件下，牛奶中的FB1和FB2均无损失。一项传播研究中，给四头奶牛分别口服（1.0和5.0 mg FB1/kg体重）或静脉注射（0.05和0.20 mg FB1/kg体重）纯FB1，结果显示，无论是否用β - 葡萄糖醛酸酶/硫酸酯酶进行水解处理以释放任何结合物，牛奶中均未检测到FB1或AP1的残留。
