Richards G E, Morrow D A, Thominet J L, Silverman B L, Gluckman P D
Department of Pediatrics, Northwestern University Medical School, Childrens' Memorial Hospital, Chicago, Illinois.
J Dev Physiol. 1993 Apr;19(4):165-9.
To test the hypothesis that growth hormone gene messenger RNA abundance in the fetus is subject to the same effects of thyroid hormone previously demonstrated in other situations, we evaluated the effect of thyroidectomy on pituitary GH mRNA content at three gestational ages in the ovine fetus. One of each twin pair of fetal lambs underwent thyroidectomy at 90, 100 or 110 days gestation. Fetal pituitaries were collected 25-30 days later. Plasma GH and IGF-I were measured as well as pituitary GH mRNA content. Serum growth hormone in the thyroidectomy group was less than in the control twins (129.8 vs 187.6 micrograms/l, P = 0.0. GH mRNA was likewise decreased in pituitaries of thyroidectomy fetuses compared to controls (1.01 vs 1.80 units, P = 0.0006). Serum IGF-I and body weight were similar in the thyroidectomy and control twins. We conclude that the ovine fetus in the final trimester of gestation exhibits effects of thyroid hormone on serum GH and mRNA abundance similar to those seen in postnatal animals.
为了验证胎儿生长激素基因信使核糖核酸丰度受甲状腺激素影响这一假说，该影响与之前在其他情况下所证实的相同，我们评估了甲状腺切除术对绵羊胎儿三个胎龄时垂体生长激素信使核糖核酸含量的影响。每对双胞胎羔羊中的一只在妊娠90、100或110天时接受甲状腺切除术。25 - 30天后收集胎儿垂体。检测血浆生长激素和胰岛素样生长因子 - I以及垂体生长激素信使核糖核酸含量。甲状腺切除术组的血清生长激素低于对照双胞胎（129.8对187.6微克/升，P = 0.0。与对照组相比，甲状腺切除术胎儿垂体中的生长激素信使核糖核酸同样减少（1.01对1.80单位，P = 0.0006）。甲状腺切除术组和对照组双胞胎的血清胰岛素样生长因子 - I和体重相似。我们得出结论，妊娠晚期的绵羊胎儿表现出甲状腺激素对血清生长激素和信使核糖核酸丰度的影响，这与出生后动物的情况相似。
